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Here, we review the current. In summary, we could selectively expand nk cells with this in vitro culture system, the expanded nk cells gained higher expression of ncrs and nkg2d receptors, and. In an attempt to set up a standardized protocol for nk cell isolation and expansion, we present a thoroughly tested and validated protocol that can produce highly pure, viable,.

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Our system demonstrated a stable expansion system without donor differences and a high expansion efficiency on days 14 and 21 and high purity in all tested cells. Many downstream applications require expansion of nk cells prior to analysis There are two primary approaches to nk cell expansion

In vivo and ex vivo